The low-grade chronic inflammtion is common in PCOS patients, who are characterized by hyperandrogenism or hyperandrogenemia＃The clinical researches have proved that the hyperandrogenemia is closely correlated with the low-grade chronic inflammtion, but the mechanism is unclear＃ Adipocyte as newly discoved inflammatory cells, When by a certain external stimulation, its inflammatorysignal transduction cascade system activated, could produce more proinflammatory mediators, such as Interleukin-6 ＃IL-6＃ and Macrophage chemotactic protein-1 (MCP-1) ＃The aim of this study was to evaluate the effects of testosterone on innate signaling and expression of pro-inflammatory mediators in 3T3-L1 adipocytes induced or not by lipopolysaccaride＃LPS＃＃The effects of testosterone on expression of proinflammatory mediators, nuclear factor-kappa-B＃NF-κB＃, and extracellular signal-regulated kinase1/2＃ERK1/2＃ signaling pathways were examined by Enzyme-Linked Immunosorbent Assay＃ELISA＃, reverse transcriptase-polymerase chain reaction＃RT-PCR＃, Western blotting and electrophoretic mobility shift assay ＃EMSA＃＃Testosterone could induce IL-6 and MCP-1, and enhance LPS-induced IL-6 and MCP-1, while the effects was not simply additive ＃ Testosterone could induce phosphorylation of ERK1/2 and NF-κB ＃The enhancement effect of testosterone on IL-6 and MCP-1 was inhibited by PD98059＃ERK1 / 2 inhibitor＃ and PDTC ＃NF-kB inhibitor＃＃ These results indicate that testosterone enhances LPS-induced IL-6 and MCP-1 expression by activating ERK1/2/NF-κB signaling pathways in 3T3-L1 adipocytes.