【摘 要】
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The fixed dynamic range of traditional biosensors limits their utility in several real applications.For example,viral load monitoring requires the dynamic range spans several orders of magnitude; wher
【机 构】
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Division of Physical Biology & Bioimaging Center,Shanghai Synchrotron Radiation Facility,Shanghai In
【出 处】
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2016年分析化学前沿国际研讨会及中美分析化学研讨会
论文部分内容阅读
The fixed dynamic range of traditional biosensors limits their utility in several real applications.For example,viral load monitoring requires the dynamic range spans several orders of magnitude; whereas,monitoring of drugs requires extremely narrow dynamic range[1].To overcome this limitation,here,we devised tunable biosensing interface using allosteric DNA tetrahedral bioprobes to tune the dynamic range of DNA biosensors.Our strategy takes the advantage of the readily and flexible structure design and predictable geometric reconfiguration of DNA nanotechnology.We reconfigured the DNA tetrahedral bioprobes by inserting the effector sequence into the DNA tetrahedron,through which,the binding affinity of DNA tetrahedral bioprobes can be tuned.As a result,the detection limit of DNA biosensors can be programmably regulated.The dynamic range of DNA biosensors can be tuned(narrowed or extended)for up to 100-fold.Using the regulation of binding affinity,we realized the capture and release of biomolecules by tuning the binding behavior of DNA tetrahedral bioprobes.
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