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Background: Varicocele (VA) is the most commonly reported correctable cause of male infertility.However, the mechanism is still far from clear.Increasing evidence suggests that seminal microbiotamight play an important role in seminal quality.Therefore, we want to know whether patients with varicocele have a changed seminal microbiota.Objective: To demonstrate whether the unbalanced seminalmicrobiome is associated with low seminal quality in patients withvaricocele.