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Fungi had become the main resource of polyunsaturated fatty acids, expecially linoleic acid.The research studied the effects and mechanism of selenium on polyunsaturated fatty acids of Diasporangiumjonesianum.The results showed that selenium could significantly increase the yields of linoleic acid.In contrast, the growth and γ-linolenic acid yield of Diasporangium jonesianum was decreased under selenium treatments.△6-fatty acid desaturase gene of Diasporangium jonesianum was investigated in this research.Sequence analysis indicated that this cDNA sequence encoded 235 amino acids.The conserved region of △6-fatty acid desaturase included three conserved histidine-rich domain, hydropathy profile, and was rich in disulfide bonds.This study showed that selenium may in-discriminatively substitute S, and incorporate selenium-amino acids into the desaturase that the conformation of enzyme active sites was impacted which leaded to the inhibition of the convert of linoleic acid to γ-iinolenic acid and the over accumulation of linoleic acid.Selenium might enhance the fatty acids contents of fi1ngi through influencing the desaturase structure.