Cooperative assembly of RecA on ssDNA revealed by capillary electrophoresis-laser induced fluorescen

来源 :43rd International Symposium on High Performance Liquid Phas | 被引量 : 0次 | 上传用户:jyin_studio
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E.coliRecA protein in theform of nucleoprotein filaments play important roles in SOS response,SOS mutagenesis and recombination repair.Here we combined highly efficient separation of capillary electrophoresis,highly sensitive laserinduced fluorescence and molecular rotation-related polarization measurements to evaluate the cooperative assembly of RecA on ssDNA in solution without ATP.
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