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Objectives In vitro maturation (IVM) has proven to be a successful method for reducing the incidence of severe ovarian hyperstimulation syndrome (OHSS) in polycystic ovary (PCO) and polycystic ovarian syndrome(PCOS) patients during in vitro fertilization (IVF) IVM takes place in period in which imprinting established, which may induce epigenetic alteration, dysregulation of gene expression and, ultimately, embryo defects or loss.To date, nothing concerning the molecular nature of epigenetic inheritance related to IVM has been reported.The aim of this research was to investigate the effects of IVM on the expression of the imprinted genes of the offspring, and to reveal possible regulatory mechanisms for these genes.Materials and methods using the IVM mice model, parts of the imprinted genes related to brain development were chosen and verified by the realtime RT-PCR.The important DNA methylation status of CpG islands of the imprinted genes H19, Kcnq1ot1 and Snrpn were detected by Bisufite Sequence PCR (BSP) Results mRNA expression of H19 was detected down-regulated and Kcnq1ot1 was detected up-regulated in IVM offspring.By using the bisulfite sequence PCR,the differentially methylated regions (DMRs) of H19 was found to have higher DNA methylation levels in IVM group compared with the control group; in contrast, Kcnq1ot1 showed a significant reduction in DNA methylationin IVM mice.However, Snrpn showed no significant differences in expression of mRNA or DNA methylation levels between the IVM and control groups.Conclusion IVM could affect the expressions of some imprinted genes in the brains of IVM offspring, which could be induced by DNA methylation alterations.