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目的:用系统血液快速固有免疫反应体外系统模式数字化实验研究探讨菌苗治疗淋巴瘤的系统免疫学机理。方法:灭活大肠杆菌(3×108/mL)0.2mL加0.6mL淋巴瘤患者枸橼酸抗凝新鲜血,对照组用生理盐水0.2mL取代大肠杆菌悬液加0.6mL全血,两管混匀37℃1h后,用流式细胞仪直接荧光免疫法测定实验组与对照组各种血细胞CD55和CD59分子表达量(平均荧光强度),并算出大肠杆菌对CD55和CD59分子表达的调控率,求出t值。结果:实验组粒细胞CD55分子的调控率为负(35.18±12.17)%,而血细胞(包括红细胞、血小板、粒细胞、淋巴细胞)CD59分子调控率为正值,以及除粒细胞CD55分子外的其它的细胞CD55分子调控率都为正值,与粒细胞CD55分子负调控率差异非常显著(P<0.05～0.01)。结论:从体外系统模式数字化实验研究证明菌苗可全方位激活系统血液免疫反应,具体机理尚待深入研究。
OBJECTIVE: To investigate the systemic immunological mechanism of bacterin vaccine in the treatment of lymphoma by using in vitro system model digitized experimental study of systemic rapid immune response in blood. Methods: Fresh blood of citric acid anticoagulation was inactivated by 0.2 mL of Escherichia coli (3 × 108 / mL) and 0.6 mL of lymphoma. The control group was replaced by 0.2 mL of saline and 0.6 mL of whole blood, After homogenized at 37 ℃ for 1 hour, the expression of CD55 and CD59 (average fluorescence intensity) of various blood cells in experimental group and control group were determined by flow cytometry direct fluorescence immunosorbent assay, and the expression rates of CD55 and CD59 on Escherichia coli were calculated. Find the value of t. Results: The regulation rate of granulocyte CD55 molecules in the experimental group was negative (35.18 ± 12.17)%, while the positive rate of CD59 molecules in blood cells (including erythrocytes, platelets, granulocytes and lymphocytes) The other cell CD55 molecular regulation rate is positive, and the negative regulation rate of granulocyte CD55 molecule is very significant (P <0.05 ~ 0.01). Conclusion: From the in vitro system model digitization experimental study proves that the vaccine can activate the systemic immune response in all aspects. The exact mechanism remains to be further studied.