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目的建立2,4,6-三硝基苯磺酸(TNBS)诱导的BALB/c小鼠炎症性肠病(IBD)模型,探讨其影响因素。方法将48只BALB/c小鼠随机分为8组:乙醇非夹闭对照组、乙醇夹闭对照组、低剂量非夹闭组、低剂量夹闭组、中剂量非夹闭组、中剂量夹闭组、高剂量非夹闭组、高剂量夹闭组,每组6只。各组分别用相应试剂灌肠造模,利用DAI评分及Wallace评分并结合一般情况评价模型。结果一般情况显示夹闭各组死亡小鼠均有肠梗阻现象,非夹闭组死亡小鼠未见肠梗阻现象;非夹闭各组体重低于乙醇非夹闭对照组(P<0.01);中剂量夹闭组和高剂量夹闭组体重低于乙醇夹闭对照组(P<0.01);高剂量夹闭组体重低于高剂量非夹闭组(P<0.01)。疾病模型评价显示中剂量非夹闭组和高剂量非夹闭组第7天DAI评分及Wallace评分均高于乙醇非夹闭对照组(P<0.01),中剂量夹闭组和高剂量夹闭组第7天DAI评分及Wallace评分均高于乙醇夹闭对照组(P<0.01);中剂量夹闭组第7天DAI评分高于中剂量非夹闭组(P<0.05);中剂量夹闭组和高剂量夹闭组Wallace评分高于同等剂量的非夹闭组(P<0.01);中剂量夹闭组Wallace镜下评分高于中剂量非夹闭组(P<0.01)。结论 TNBS诱导BALB/c小鼠IBD模型过程中,在造模试剂注入结肠后不宜夹闭肛门。同时,相对于大鼠,小鼠造模所用的TNBS剂量略高。
OBJECTIVE: To establish a BALB / c mouse model of inflammatory bowel disease (IBD) induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) and to explore its influencing factors. Methods 48 BALB / c mice were randomly divided into 8 groups: non-occluded alcohol control group, ethanol occlusion control group, low-dose non-occlusion group, low-dose occlusion group, middle-dose non-occlusion group, Clamping group, high-dose non-clamping group, high-dose clamping group, 6 rats in each group. Each group were enema with the corresponding reagent modeling, the use of DAI score and Wallace score combined with the general situation evaluation model. Results The general situation showed that all the dead mice in each group had intestinal obstruction. No intestinal obstruction was found in the non - occluded group. The body weight in non - clipped groups was lower than that in the non - occluded group (P <0.01). The body weight of middle-dose group and high-dose group were lower than that of ethanol-occlusion group (P <0.01). Body weight of high-dose group was lower than that of high-dose group (P <0.01). The disease model evaluation showed that DAI score and Wallace score on the 7th day in the medium-dose non-occlusion group and the high-dose non-occlusion group were higher than those in the non-occlusion group (P <0.01), middle-dose group and high-dose occlusion group DAI score and Wallace score on the 7th day were higher than those in the ethanol occlusion control group (P <0.01). The DAI score on the 7th day in the middle dose group was higher than that in the non-occlusion group (P <0.05) The Wallace scores in the closed and high-dose groups were higher than those in the non-occluded group (P <0.01). The Wallace scores in the medium-dose group were higher than those in the non-occluded group (P <0.01). Conclusion TNBS induced BALB / c mouse IBD model during injection of the model agent into the colon should not close the anus. At the same time, mice were modeled at a slightly higher dose of TNBS relative to the rats.