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Objective To investigate the influence of TACE and autologous cytokine- induced killer (CIK) cells on the peripheral T lymphocyte subsets and native killer cell in patients with advanced primary hepatocellular carcinoma(HCC).Method Methods A total of 136 patients with unresectable HCC were divided into study group(n=67,patients were treated with CIK cell therapy after TACE),and control group(n=69,patients were treated with TACE only).