【摘 要】
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Objective: To study on homology and evolution of the methylated regions of hepatitis B viral strains from Guangdong area.Methods: Sixty entire gene sequences that were delivered by Guangdong area were
【机 构】
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Research Center for Reproductive Medicine, Shantou University Medical College, Shantou 515041, China
【出 处】
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3rd Asian Conference on Environmental Mutagens & 15th Confer
论文部分内容阅读
Objective: To study on homology and evolution of the methylated regions of hepatitis B viral strains from Guangdong area.Methods: Sixty entire gene sequences that were delivered by Guangdong area were gotten from International Nucleic Acid Databases (GenBank\EBI\DDBJ) and Hepatitis Virus Database (HVDB).The methylated regions of the 60 gene sequences were analyzed by methprimer online-software, and then the evolution tree of these sequences and their methylated regions were established by using biosoftware ClustalW 1.83 and Mega 4.0.Results: There are three methylated regions in HBV sequences from Guangdong viral strains : 68-286bp, 1116-1725bp, 2070-2466bp, and one mutant methylated region (330-595bp) only happens in several HBV sequences.And, there are significant differences in 68-286bp and 1116-1725bp methylated regions.Furthermore, there are extensive sequence divergences between methylated regions in HBV genes.Conclusion: The methylated regions of hepatitis B viral strains from Guangdong area display large heterogeneity, and the differentially methylated regions of the same virus also showed the divergence rate.
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