[Objective] To investigate the thrombolytic and antiplatelet effects of a novel plasminogen activator from the venom ofGloydius brevicaudusviper (GBV-PA) in vitro and in vivo.[Methods] Thrombolytic experiments were performed in rabbit models of ear vein thrombosis and carotid artery thrombosis, and in dog model of acute cerebral infarction.Inhibition of thrombus formation was evaluated in rat inferior vena cava thrombosis model and ferric chloride-induced arterial thrombosis.