论文部分内容阅读
the present study,we investigated the anti-nociceptive effect and the underlying mechanism of the analgesic-antitumor peptide (AGAP),a neurotoxin from the scorpion Buthus martensii Karsch.AGAP in doses of 0.2,1 and 5 μg was intraplantar (i.pl.) injected before formalin injection 10 min at the same site.The suppression by intraplantar injection of AGAP on formalin-induced spontaneous nociceptive behaviors was investigated.The results showed that AGAP could dose-dependent inhibit formalin-induced two-phase spontaneous flinching response.To investigate the mechanism of action of treatment with AGAP in inflammatory pain,the expression of peripheral and spinal phosphorylated mitogen-activated protein kinases (phospho-MAPKs) including p-p38,p-ERK and p-JNK were examined.We found that formalin increased the expression of peripheral and spinal MAPKs,which were prevented by pre-intraplantar injection of AGAP in inflammation pain model in mice.Furthermore,AGAP could also decrease the expression of spinal Fos induced by formalin.The present results indicated that pre-intraplantar injection of AGAP prevented the inflammatory pain induced by formalin through a MAPKs-mediated mechanism in mice.