【摘 要】
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Due to easy primer design and lower sequencing costs,the 16S rRNA gene is commonly targeted and sequenced in microbiome studies in order to survey the phylogenetic content of a sample.However,it has b
【机 构】
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School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia,USA
【出 处】
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第四届国际人体微生态大会、第十三届中华预防医学会微生态学学术会议、第五届中国医师协会感染科医师大会暨传染病诊治高峰论坛、
论文部分内容阅读
Due to easy primer design and lower sequencing costs,the 16S rRNA gene is commonly targeted and sequenced in microbiome studies in order to survey the phylogenetic content of a sample.However,it has been of-interest to look at other housekeeping genes that are nearly universal to all prokaryotes (a.k.a marker genes),especially since multiple markers may be present whole-genome shotgun samples [1,2].However,the selection of markers may reveal slightly different phylogenies,as different genes evolve at different rates and may recover better or worse the phylogenetic relationships of different lineages.However,choosing markers is often complicated with insufficient knowledge of the genes and can be time consuming to verify all possibilities.
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