It is standard practice to study GVHD in lethal irradiation-based murine models.Frequently,major histocompatibility (MHC) mismatched donors are used in GVHD models.In contrast,in clinical allo-HSCT conditioning with chemotherapy (+/-irradiation) is common and donors are often MHC-matched.Aiming at a more clinically oriented situation,we established and characterized a murine MHC-matched,minor HA-mismatched GVHD model (LP/J[H2kb]→ C57BL/6[H2kb]) using Busulfan and Cyclophosphamide conditioning.We found typical clinical and histological features of acute GVHD.T cell infiltration and GVHD-specific damage emerged in the skin,gastrointestinal tract,liver and lymphoid organs during GVHD progression.Systemic inflammation involving changes in immune cell subsets and cytokine profile was similar to observations made in patients after allo-HSCT.