论文部分内容阅读
Swine mycoplasmal pneumonia (SMP), caused by Mycoplasma hyopneumoniae (Mhp), is one of the most important respiratory diseases in swine breeding.The objective of this study was to develop a recombinant subunit vaccine rLTBR1 containing R1 region of p97 adhesin of M.hyopneumoniae (Mhp) and B subunit of the heat-labile enterotoxin of Escherichia coli (LTB).The result indicated that rLTBR1 revealed GM1 ganglioside-binding capacity.The immunogenicity of rLTBR1 was detected through BALB/ c mice.The results suggested that inoculated rLTBR1 through the intranasal (IN) or intrasubcutaneous (IS) route both produced specified anti-Mhp systemetic and mucosal antibody (sIgA).The group inoculated R1 by IS route also produced specified anti-Mhp systemetic antibody, but through the IN route didnt induce specified and mucosal antibody.Group administrated rLTBR1 through the IN route also induced IFN-γ secretion by lymphocytes.Howerver, the other groups didnt induced IFN-γ compared to the negative group.In conclusion, LTB can enhance the mucosal and cellular immunity level through the IN route.Certainly, the immune responses of mice should not be extrapolated, so the potential of the rLTBR1 for the control of SMP requires further studies in pigs.This study suggested that rLTBR1 vaccine may establish a new strategy for preventing infection from M.hyopneumoniae.