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5. 蟑螂粗提物对人支气管上皮细胞蛋白酶活化受体-2及IL-6和8分泌的影响

蟑螂粗提物对人支气管上皮细胞蛋白酶活化受体-2及IL-6和8分泌的影响

来源 :2014中华医学会呼吸病学年会暨第十五次全国呼吸病学学术会议 | 被引量 : 0次 | 上传用户：ytw1234

【摘 要】

：

目的 观察德国小蠊粗提物(GCE)对人支气管上皮细胞(HBE-16)蛋白酶活化受体-2(PAR-2)表达及IL-6、8分泌的影响.方法 实验组暴露于GCE (25、50、75 μg/ml)、加或不加TNF-α (3 ng/ml) 24 h.在GCE+TNF-α组中加入PAR-2激动剂SLIGKV-NH2或PAR-2小干扰RNA.蛋白质印迹法、免疫荧光法检测PAR-2表达.ELISA检测细胞上清IL

【作 者】

：

刘洪泱 谢林艳 陆娇 闫兆 马元 解卫平 黄茂 周林福 

【机 构】

：

南京医科大学第一附属医院呼吸科 南京医科大学第一附属医院呼吸科;江苏盛泽医院(江苏省人民医院盛泽分

【出 处】

：

2014中华医学会呼吸病学年会暨第十五次全国呼吸病学学术会议

【发表日期】

：

2014年3期

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目的 观察德国小蠊粗提物(GCE)对人支气管上皮细胞(HBE-16)蛋白酶活化受体-2(PAR-2)表达及IL-6、8分泌的影响.方法 实验组暴露于GCE (25、50、75 μg/ml)、加或不加TNF-α (3 ng/ml) 24 h.在GCE+TNF-α组中加入PAR-2激动剂SLIGKV-NH2或PAR-2小干扰RNA.蛋白质印迹法、免疫荧光法检测PAR-2表达.ELISA检测细胞上清IL-6、IL-8和VEGF水平.实时荧光PCR检测IL-6、IL-8和VEGF基因表达.

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