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  5. Biological Characteristics of Osteogenic and Angiogenic Bi-lineage Differentiated ADSCs Sheet-implan

Biological Characteristics of Osteogenic and Angiogenic Bi-lineage Differentiated ADSCs Sheet-implan

来源 :2014xi`an Conference of International Society for Maxillofac | 被引量 : 0次 | 上传用户：new_spider
【摘 要】
：
Purpose: This study aimed to construct osteogenic and angiogenic bi-lineage differentiated adipose tissue-derived stem cells(ADSCs)sheet-implant complex and investigate its biological characteristics.
【作 者】
：
Nan Ren Yan Dong Wei Zhou Huan Liu Yimin Zhao 
【机 构】
：
Department of Prosthodontics,School of Stomatology,Fourth Military Medical University,Xi'an 710032
【出 处】
：
2014xi`an Conference of International Society for Maxillofac
【发表日期】
：
2014年4期
【关键词】
：
Adipose tissue-derived stem cells Osteogenic Angiogenic Cell sheet Implant 
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　　Purpose: This study aimed to construct osteogenic and angiogenic bi-lineage differentiated adipose tissue-derived stem cells(ADSCs)sheet-implant complex and investigate its biological characteristics.Method: The osteogenic and angiogenic bi-lineage differentiated ADSCs sheet(Group A)and osteogenic differentiated ADSCs sheet(Group B)were warped on traditional pure Ti implant separately and the obtained complexes were cultured in vitro for 4weeks.ALP activity and the expression of BMP-2 and VEGF of the complex surface were investigated after 1d,14d and 28d of culture.Then the complexes were implanted into subcutaneously pockets of severe combined immunodeficiency mice.After 8 weeks transplantation,Micro CT assay was conducted in vivo.Then recover the complex,strip the tissue around it and stain with HE to observe its microstructure.
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