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Purpose: This study aimed to construct osteogenic and angiogenic bi-lineage differentiated adipose tissue-derived stem cells(ADSCs)sheet-implant complex and investigate its biological characteristics.Method: The osteogenic and angiogenic bi-lineage differentiated ADSCs sheet(Group A)and osteogenic differentiated ADSCs sheet(Group B)were warped on traditional pure Ti implant separately and the obtained complexes were cultured in vitro for 4weeks.ALP activity and the expression of BMP-2 and VEGF of the complex surface were investigated after 1d,14d and 28d of culture.Then the complexes were implanted into subcutaneously pockets of severe combined immunodeficiency mice.After 8 weeks transplantation,Micro CT assay was conducted in vivo.Then recover the complex,strip the tissue around it and stain with HE to observe its microstructure.