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Microfluidic devices can be utilized to combine injection, separation, and detection, without the need for traditional unions; thus, enabling the analysis with greatly reduced extra column band broadening.Reducing dispersion due to unions potentially allows fast and ultra-fast analyses while maintaining high chromatographic performance.However, as the dimensions of a chromatographic column decrease the corresponding peak volumes decrease proportionally which places increases demands on reducing extra-colUmn band broadening.This demand is further compounded when performing fast analyses with ballistic gradients.When detecting sharp peaks of small volume any source of post-column dispersion is detrimental to achievable peak capacity.Using a chromatographic theory of dispersion we investigated the effect of dispersion on chromatographic performance in isocratic and gradient mode for multiple microfluidic chromatographic devices.The calculated results are compared with experimental chromatograms for ceramic and titanium based microfluidic chromatographic devices packed with 1.7 and 2.5 μm C 18 sorbent.Implications of pre-and post-column dispersion in injector, detector, and connecting tubing on separation performance are discussed.