In-situ transmission electron microscopy (TEM) method is powerful in a way that it can directly corre late the atomic structure with physical and chemical properties.In this presentation, we will report on our progress on the construction and research applications of the in-situ TEM-SPM system for nanomanipulation and nanomeasurement.The properties at nanoscale un der various physical stimuli have been studied inside TEM, including electrical, optical, optical-electrome chanical coupling properties.Recently, the atomic scale observation of ion immigration and their related structural transformation and electrochemical behavior has been achieved inside TEM, and the electrochemi cal process involved mass transfer was also imaged,such that the mechanism of catalysis, resistive switc hing memories and lithium ion batteries could be un derstood in several nanostructures.