A series of Ofloxacin imidazole hybrids as potentially antimicrobial agents have been successfully synthesized via multistep reactions starting from commercial ethyl acetoacetate and triethylorthoformate.This synthetic route mainly involved cyclization and nucleophile substitution reactions,which has several advantages including commercially available starting materials,easy operation and moderate to good yield.The antibacterial evaluation revealed that most of the synthesized compounds exhibited good antibacterial efficiencies.Especially,compound 3a displayed superior anti-bacterial activity against Escherichia coli ATCC 25922 with MIC value of 0.023 μmol/mL.