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Polyeyclic aromatic hydrocarbons (PAHs) are compounds of intense public concern due to their persistence in the environment and potentially deleterious effects on human, environmental and ecological health.Benzo (a) pyrene (BaP) is a five-ring polycyclic aromatic compound with acute carcinogenicity, widely distribution and stablely property.It is classified as a priority pollutant in environment assessment.Biodegradation is a significant tool for the removal of PAHs from environments.N.pentaromativorans US6-1 is an exclusive bacterium that growing with Bap as sole carbon and energy sources, its Genome wide has been published.Some flagellated bacteria have specific systems to detect and respond behaviorally to pollutants present in aerobic environments by the process of ehemotaxis, and chemotaxis can enhance biodegradation.To investigate chemotaxis and transport mechanism of PAHs in N.pentaromativorans US6-1, the transcriptome of the N.