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为了研究黄葵黄酮类成分的肠吸收机制,进行了黄葵醇提物不同浓度、不同肠段大鼠在体肠吸收研究。采用HPLC法测定循环液中药物浓度,分析了黄葵6种黄酮类成分的肠吸收,开展了P-gp抑制剂对黄葵黄酮类成分吸收影响的研究。结果显示:黄葵各黄酮成分在不同浓度下,Ka值没有明显差异(P>0.05),吸收呈一级动力学过程,提示为被动扩散吸收;各黄酮成分之间的吸收有差异性,苷类成分的Ka值小于苷元类成分;各成分在不同肠段均有吸收,金丝桃苷和杨梅素的最佳吸收部位分别为空肠和十二指肠;P-gp抑制剂维拉帕米可促进异槲皮苷、金丝桃苷、杨梅素和槲皮素3’-O-葡萄糖苷的吸收。
In order to study the mechanism of intestinal absorption of flavonoids, the effects of different concentrations of alcohol extract of Houttuynia cordata and different intestinal segments on intestinal absorption in vivo were studied. The drug concentration in circulating fluid was determined by HPLC. The intestinal absorption of six flavonoids in Huang Kui was analyzed and the effect of P-gp inhibitor on the absorption of flavonoids in Huang Kwai was studied. The results showed that there was no significant difference in the Ka value (P> 0.05) between the flavonoids of Huangkuo and the first-order kinetics, which suggested that they were absorbed by passive diffusion. The absorption of flavonoids was different, Ka-like component is less than aglycone components; each component in different segments of the intestinal absorption, hyperoside and myricetin best absorption sites were jejunum and duodenum; P-gp inhibitor verapam Rice can promote the absorption of isoquercetin, hyperin, myricetin and quercetin 3’-O-glucoside.