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To determine the genomic structure of a potato virus Y (PVY) isolate from Yulin, China and its phylogenetic relationship with reported PVY strains.The complete genome of the PVY ShX14 was amplified and sequenced from overlapping fragments using 11 pairs of primers designed from the conserved regions of the known PVY isolates.Genomic structure and recombination events of the isolate were evaluated by various bioinformatics approaches.Phylogenetic tree was reconstructed by Maximum Likelihood method (ML) using nucleotide sequences of coding regions.In addition, phylogeny-trait association analysis was used to evaluate the relationship between PVY isolate and known stains reported.The results show that the complete sequence of ShX14 has 9, 724 nucleotides, excluding the 3-terminal poly (A) tail.