Objective: This study aimed to use the voxel-mirrored homotopic connectivity(VMHC)to explore if there are neuroimaging changes in college students with smartphone addiction(SA).SA has become a prevalent issue,especially,in college students recently.Students with SA become unsocial,irritable,inattentive,out of control,and exhibit many other behavioral changes.The resting-state functional magnetic resonance imaging(rs-fMRI)is a hot approach to study the functional connectivity of brain.VMHC is a newly developed method based on rs-fMRI to investigate the interhemispheric resting-state functional connectivity of the whole brain.Methods: All participants,aged 20–26 years,were recruited from the Peoples Hospital of Zhengzhou University,China.Twentyfour participants with SA and 16 healthy controls,age-,gender-,and education-matched,were imaged.All participants underwent a resting-state functional and structural magnetic resonance imaging.An automated VMHC approach was used to analyze the data.A two-sample t test was used to compare different VMHC values of the brain regions of SA participants with those of control participants.Finally,significantly altered brain regions were adopted as regions of interest for correlation analyses.Results: The SA participants showed decreased VMHC than healthy controls in the cuneus and posterior cingulate cortex.Further analyses showed significant negative correlation between VMHC in the posterior cingulate cortex and mobile phone addiction index.Conclusions: This study demonstrated decreased VMHC in the cuneus and posterior cingulate cortex in the participants with SA.The VMHC could be a biomarker for assessing the pathological change in brain due to SA.