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  5. The establishment of the duplex real-time RT-PCR assay for the detection of CD44v6 in pancreatic can

The establishment of the duplex real-time RT-PCR assay for the detection of CD44v6 in pancreatic can

来源 :国际(广州)癌症治疗论坛 | 被引量 : 0次 | 上传用户：mn666666
【摘 要】
：
Cell adhesion molecule CD44v6 has been found to be associated with the progression and metastasis of numerous cancers.In this study, a novel duplex real-time quantitative reversetranscription PCR (qRT
【作 者】
：
Gang Zhou David Chiu Dajiang Qin Lizhi Niu Jinlei Cai Lihua He Wenhao Huang Kecheng Xu 
【机 构】
：
Department of Oncology,The GIBH Affiliated Fuda Hospital,Chinese Academy of Sciences,Guangzhou,Peopl
【出 处】
：
国际(广州)癌症治疗论坛
【发表日期】
：
2013年4期
【关键词】
：
CD44v6 quantitative real-time RTPCR pancreatic cancer 
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　　Cell adhesion molecule CD44v6 has been found to be associated with the progression and metastasis of numerous cancers.In this study, a novel duplex real-time quantitative reversetranscription PCR (qRT-PCR) assay was developed to quantitatively detect the CD44v6 gene expression in pancreatic cancer patients.The primers and probes of Cd44v6 and β-actin genes were designed and standard curve of the duplex qRT-PCR was constructed by optimizing the reaction conditions.
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