论文部分内容阅读
AIM To investigate the proliferation-inhibiting effects and mechanisms of the combination of an active ingredient from Rabdosia rubescens GP and paclitaxel (PTX) on human gastric cancer MGC80-3 cells in vitro.METHODS After administration of GP (0.5-4 μmol· L-1) or PTX (1-8 nmol· L-1), or the combination for 48 h, MTT assay was used to detect the inhibition rates and the drug combination was determined by Chou-Talalay method through calculating combination index (CI) values.The expression of p53 was evaluated by Western blot.RESULTS The inhibition rates of combination groups were remarkablely higer than that of the respective single-agent groups.When the inhibition rate was higer than 30%, the combination of GP and PTX produced the CI below 1, suggesting a synergy between GP and PTX in inhibiting cell proliferation.Westem blot results showed that the combination treatment caused an increased expression of p53 in a dose-dependent manner compairing to either GP or PTX alone.CONCLUSION GP synergistically enhanced the prohferation-inhibiting effect of PIX in human gastric cancer MGC80-3 cells in vitro and the potential mechanism may be the activation of p53 pathway.