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The goal of our study was to raise monoclonal antibodies (mAbs) acgainst colonic carcinoma.Here, we describe the generation and intensive characterization of rmAb 3P9, including its functional properties and its antigen identification.In our study, the IgM isotype mAb 3P9 showed remarkably restricted immunoreactivity in colonic carcinoma.We identified the mAb 3P9 antigen as a disaccharide, sialyl-Tn, a cancer-associated carbohydrate antigen expressed in cancers of the digestive tract.In eddition, mAb 3P9 inhibits the migration of HCT-8 cell line and induces apoptosis of LSC (sTn-positive cell line), inhibits the growth of human xenograft tumors by inducing apoptosis in mice.Our data provide a novel anti-sTn mAb 3P9 that could inhibit the human tumor growth in mice and demonstrate that mAb 3P9 has potential as a diagnostic and therapeutic agent in colonic cancer biology.