【摘 要】
:
5-hydroxymethylcytosine(5hmC)is a recently re-discovered DNA base that is converted from the well-characterized epigenetic mark 5-methylcytosine(5mC).The le
【机 构】
:
StateKeyLaboratoryofEnvironmentalChemistryandEcotoxicology,ResearchCenterforEco-EnvironmentalScience
【出 处】
:
第九届全国微全分析系统学术会议、第四届全国微纳尺度生物分离分析学术会议、2014国际微流控芯片与微纳尺度生物分离分析学术
论文部分内容阅读
5-hydroxymethylcytosine(5hmC)is a recently re-discovered DNA base that is converted from the well-characterized epigenetic mark 5-methylcytosine(5mC).The level of 5hmC has been observed to be altered in several diseases,including hematopoietic malignancies and a broad range of solid tumours(1,2).A lack of 5hmC was implicated as a useful biomarker for cancer diagnosis(3).Interestingly,both nutritional(e.g.,vitamin C,4)and environmental factors(e.g.,redox-active quinones,5)affect the cellular level of 5hmC.These observations may indicate that 5hmCvarying or-rich genes or sequences could be exploited as indicators or biomarkers of epigenetic states,pathogenic processes,pharmacological responses,and environmental exposures.Here,we find that boronic acid(BA)can inhibit the amplification activity of Taq DNA polymerase for replicating glucosylated 5hmC bases in template DNA by interacting with their glucose moiety.On the basis of this finding,we propose for the first time a BA-mediated qPCR assay for rapid and sensitive detection of gene-or fragment-specific 5hmC without restriction-assay-like sequence limitations.Using the optimized assay,we demonstrate the enrichment of 5hmC in an intron region of Pax5 gene(a member of the paired box family of transcription factors)in mouse embryonic stem(ES)cells.Our work potentially opens a new way for the screening and identification of 5hmC-rich genes and for high throughput analysis of 5hmC in mammalian cells.
其他文献
小分子诱导胚胎干细胞(ESCs)定向分化为功能性细胞是目前的研究热点.在ESCs定向分化的体外模型中,三维球状结构的拟胚体(EBs)是常用的一种自发分化途径.传统EBs形成方法
The living system in vivo is a complex system consisting of hierarchical microenvironment with flexible architectures,with size scales spanning from the micront
Hydrogel are polymer materials with covalent or non-covalent crosslinks containing large fraction of water.Hydrogel microfluidics applies hydrogel for fluid
We demonstrated a microfluidic device for high efficiency and high purity capture of circulating tumor cells(CTCs)from peripheral blood of cancer patients.T
Mesothelin,as a kind of tumor cell surface differentiation antigen,appears in the early stage of pancreatic cancer.Therefore,it can be used as a potential n
耐甲氧西林金黄色葡萄球菌(Methicillin-Resistant Staphylococcus Aureus,MRSA)是引起院内感染的主要病原菌之一,易引起交叉感染,耐药性和致病能力强,极大地增加了临床
In this investigation,a rapid and high-throughput method for profiling of TAGs in plant oils by liquid chromatography using a single column coupled with atm
Although the universal homogeneous G-quadruplex-DNAzyme based aptasensors have potentials to replace conventional biosensors,the high background induced by
Recently,some nanoparticles are widely used as labeling probes in biomedical assay,which mainly include quantum dots(QDs),metal clusters,gold nanoparticles(
It is quite important to separate and enrich the target compounds from complicated sample matrices by suitable sample preparation techniques prior to the ch