Regulation of Drosophila circadian rhythms by miRNA let-7 is mediated by a regulatory cycle

来源 :2015年中国中西医结合学会时间生物医学专业委员会学术研讨暨换届会议 | 被引量 : 0次 | 上传用户:liongliong598
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  MicroRNA-mediated post-transcriptional regulations are increasingly recognized as important components of the circadian rhythm.Here we identify microRNA let-7, part of the Drosophila let-7-Complex, as a regulator of circadian rhythms mediated by a circadian regulatory cycle.Overexpression of let-7 in clock neurons lengthens circadian period and its deletion attenuates the morning activity peak as well as molecular oscillation.Let-7 regulates the circadian rhythm via repression of CLOCKWORK ORANGE (CWO).Conversely, up-regulated cwo in cwo-expressing cells can rescue the phenotype of let-7-Complex overexpression.Moreover, circadian prothoracicotropic hormone (PTTH) and CLOCK regulated 20-OH ecdysteroid signaling contribute to the circadian expression of let-7 through the 20-OH ecdysteroid receptor.Thus, we find a regulatory cycle involving PTTH, a direct target of CLOCK, and PTTH-driven miRNA let-7.
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