The fingerprints ofAconitura L.plants,Radix Aconiti (RA),Radix Aconiti Singularis (RAS),Radix Aconiti Kusnezoffii (RAK),Radix Aconiti Lateralis Preparata (RALP) and Radix Aconiti Brachypodi (RAB), were established by ultra-performance liquid chromatography (UPLC) and the metabolism of Aconitum L.plants on Escherichia coli (E.colti) was studied by microealarimetry.Some quantitative thermo-kinetic parameters such as the heat-flow maximum power (Pm) and the heat output (Qsta),which obtained from the thermo-genic curves of E.coli growth affected by five Aconitura L.plants.The spectrum-effect relationships between UPLC fingerprints and bacterial metabolism were investigated using canonical correlation analysis (CCA).The results showed that there were close relationships between contents of correlative chemical compositions and bioaetivities,while aconitine,hypaconitine and mesaconitine in the UPLC fingerprints might be the relevant bacterial activities components.Because of their inherent differences of chemical compositions,the main aetives of bacterial metabolism were different among five Aconitum L.plants.The potency bioactivity sequence of the tested five Aconitum L.products on E.coli was obtained that were RAB > RALP > RAK > RAS > RA.This work establishes a universal model of the combination of UPLC and microcalorimetry to study the spectrum-effect relationships ofAconitum L.plants,which offers some references for detecting principle components of traditional Chinese medicine on bioactivity.