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Objective To investigate the expression ofrmicroRNA-21 (miR-21) in breast cancer cell lines and serum of patients with breast cancer and the impact on the invasion and migration of breast cancer cells.Methods Total RNA were isolated from breast cancer cell lines by TRIzol,serum miRNAs were extracted by mirVanaTM miRNA Isolation Kit.miR-21 expression were determined by fluorescent quantity polymerase chain reaction in 4 breast cell lines (HBL-100,MCF-7,MDA-MB-231 and MDA-MB-468) and in serum from breast cancer patients (n=56) and breast benign disease patients (n=39) with health controls (n=45).The characteristics of cell invasion and migration were examined after miR-21 inhibitor transfection by lipofectamin.Results Real time FQ-PCR result indicated miR-21 high expression in breast cancer cell lines than normal breast cells.Altered expression of miR-21 was observed in the highly invasive breast cancer cells,compared with weakly invasive breast cancer cells.The expression of miR-21 was significantly increased in the breast cancer patients,compared with benign breast diseased patients and health control.But no significant change was observed on the expression of miR-21 in benign breast diseased patients compared with health control.The capacity of breast cancer cell invasion and migration was significantly decreased after transfection of miR-21 inhibitor.Conclusions MiR-21 is high expression in breast cancer cell lines and breast cancer patients serum.Altered expression of miR-21 maybe plays an important role in breast cancer invasion and migration.MiR-21 may serve as new biomarker to early detection and prognosis estimates of breast cancer.