Molecular genetic techniques have proven very useful for initial analysis of the extent of genetic variation and dispersal in several Antarctic moss species.In the present study, the small subunit ribosomal RNA (SSU rDNA) and internal transcribed spacers of the nuclear ribosomal DNA (ITS rDNA) were sequenced in nine individuals of different mosses from the Fildes Peninsula of Antarctica.Sequence alignment showed that the extreme environment tended to increase the genetic diversity of Antarctic mosses.In addition, in our phylogenetic analysis, one previously unidentified Antarctic moss species was characterized by comparison with SSU and ITS rDNA sequences of known moss species.Moreover, the optimal culture medium and conditions for surface explant sterilization and protonemata induction in tissue culture of Pohlia nutans were investigated.The successful establishment of a tissue culture protocol together with the phylogenetic analysis of Antarctic mosses will provide technological support to establish an effective resource regeneration method for discovering new functional genes and gaining novel insights into the mechanisms of stress acclimation.