【摘 要】
：
To explore the development of a vaccine against transmissible gastroenteritis virus (TGEV) infections,the gene coding for the TGEV protein(S) was inserted into the swinepox virus (SPV).We obtained a r
【机 构】
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College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China
【出 处】
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第五届中国兽药大会(中国畜牧兽医学会动物药品学分会2014年学术年会、中国畜牧兽医学会生物制药学分会暨中国微生物学会兽医
论文部分内容阅读
To explore the development of a vaccine against transmissible gastroenteritis virus (TGEV) infections,the gene coding for the TGEV protein(S) was inserted into the swinepox virus (SPV).We obtained a recombinant SPV (rSPV-SA) that expresses a TGEV protectlive antigen and that acts as a potential vaccine.Results of PCR and indirect immunofluorecence detection indicated the construction of recombinant virus was successful.For in vivo experiments,immune responses of the vaccination vector were evaluated in the mouse model.The Indirect ELISA assay suggested that when mice were vaccinated with rSPV-SA,the IgG against TGEV were dramatically enhanced particularly during the second inoculation the rSPV-SA.The Cytokine assay show that both Thl-type and Th2-type cytokine levels increased dramatically after vaccinated the rSPV-SA.The neutralization assays results indicate that serum from BALB/C mice vaccinated with rSPV-SA effectively neutralized the CPE production by TGEV.We conclude from our data that our novel recombinant SPV construct could be a potential vaccine against TGEV infection.
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