Objectives: We applied intravoxel incoherent motion(IVIM)diffusion-weighted imaging(DWI)and blood oxygen leveldependent(BOLD)imaging to detect the serial effects of iodinated contrast media(CM)on intra-renal hemodynamics,diffusion and oxygenation.Materials and Methods: Twenty-four rats received CM to induce CIAKI.Functional MRI were performed on rats(n = 6)at before and after injection to obtain D,D*,f and R2*.Three rats were sacrificed at each time point for pathologic assessment.Results: The cortical D value reached its minimum at 48 h(p = 0.027),and gradually returned at 72 h(p = 0.027),while the medullary D value decreased until to 72 h(p = 0.027).All D* values reached their minimum at 24 h(p = 0.027~0.046),and gradually returned at 48 h(p = 0.028~0.046).f values decreased until to 48 h(p = 0.028~0.075)and then ascended at 72 h(p = 0.028~0.075).The outer medullary R2* values prominently increased at 30 min(p = 0.028),remained elevated until to 48 h(p = 0.028),but returned to baseline at 72 h(p = 0.028).Regression analysis showed R2* had a significant correlation with D* in the outer medulla and inner medulla(t = 2.2,p = 0.039; t = 3.9,p = 0.001,respectively)but not in the cortex(t =-1.40,p = 0.18).The histology assessment confirmed the progressive,pathological changes.Conclusions: IVIM DWI and BOLD MRI indicate efficay in evaluating renal hemodynamics,diffusion and oxygenation of CIAKI.