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The causative agent of toxoplasmosis,Toxoplasma gondii,can infect virtually all nucleated cell types of warm-blooded animals.In this study,we examined the sequence variation in calcium-dependent protein kinase 2(CDPK2)genes among 13 T.gondii strains from different hosts and geographical locations.Results showed that the lengths of complete CDPK2 DNA and cDNA sequences were 3671-3673 bp and 2136 bp,respectively,and the sequence variation were 0-0.9%among different T.gondii strains.Phylogenetic analysis based on the CDPK2 gene sequences revealed T.gondii strains of the same genotypes were clustered in the different clades.Further analysis of all the other T.gondii CDPK genes in genotype Ⅰ(GT1),Ⅱ(ME49)or Ⅲ(VEG)strains indicated the T.gondii CDPK gene family was quite conservative,and the sequences variation ranging from 0 to 1.40%.The study concluded that CDPK2 as well as all the CDPK genes in T.gondii cannot use as proper marker for studying the variant of different T.gondii genotypes from different hosts and geographical locations,which may be a useful feature as anti-T.gondii vaccine candidates in further studies.