Curvularia lunata (Wakker) Boed, a causal agent of corn Curvularia leaf spot, has been one of the major leaf diseases in production regions of China.In recent years, many studies mainly focused on breeding resistant varieties, occurrence investigation and identification of pathogenic differentiation.We have less understanding on relationship between nutrient element iron and pathogenicity mechanismof C.lunata.Therefore, in this study ATMT target gene knockout system of C.lunata is optimized, and as a platform to knock out the gene ClFtrl, ecoding Fe transporter permease.It is the foundation for studying ClFtrl and relationship with pathogenic, simultaneously explaining pathogenicity of C.lunata from iron metabolic, to enrich machanism of molecular pathogenesis.