Telomeres are highly ordered DNA-protein structures at the ends of linear eukaryotic chromosomes.They not only help to distinguish chromosome e nds from DNA double-strands breaks, but also provide a way to replicate chromosomes completely.Telomeric DNA can be efficiently replicated by telomerase or recombination in Saccharomycescerevisiae (budding yeast).Genome-wide screening of telomere length in non-essential gene deletion yeast strain set had identified more than 280 genes which are involved in telomere maintenance.As a complementarity, we screening the telomere lengths in additional 66 non-essential genes which previous were taken for essential genes in large-scale deletion project.