OBJECTIVE To investigate the effect of recombinant toxin protein rLj-RGD from Lampetra japonica (RGD) on growth and metastasis and lengthening life time in C57BL/6 mice bearing Lewis lung cancer.METHODS C57BL/6 mice implanted with Lewis lung cancer cells were randomly divided into five groups: model group, RGD high (0.28 mg·kg-1), middle (0.14 mg·kg-1), low (0.07 mg·kg-1) dose groups and Doxorubicin group.Two days after tumor implantation, RGD, Doxorubicin and saline were given by intraperitoneal injection daily by twice.Four weeks later, the volume and weight of subcutaneous tumors were measured, the pathological change of the tumor tissue was observed with HE staining method, in order to evaluate the anti-tumor effect of RGD on tumor growth.Four weeks later, the average number of lung metastasis clones was counted to evaluate the anti-tumor effect of RGD on tumor metastasis.Compared with model group, the hfe time prolongation rate was calculated till the mice died.RESULTS The Lewis lung cancer-bearing model was successfully established in mice.The tumor volumes and weight of the treatment groups were smaller than that of the model group (P < 0.01) and tumor size inhibition rate (P < 0.01) were 35 % , 43.9 % , 56.8 % ; the weight of tumors inhibition rate were 42.8%, 59.6%, 73.5% ; the average number of lung metastasis were reduced (P <0.01), and transfer inhibition rate were 76.5%, 94.1%, 98.5%.The life time of Lewis lung cancer-bearing mice were lengthened.CONCLUSION RGD has a significant anti-tumor effect on inhibiting primary tumor growth and metastasis and lengthening the life time of mice bearing Lewis lung cancer.