【摘 要】
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Spontaneous variants of Beet black scorch virus (BBSV) and its satellite RNA were generated from cDNA clones by serial propagation in Chenopodium amarantico
【机 构】
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State Key Laboratory of Agro-Biotechnology,China Agricultural University,Beijing 100193,China
【出 处】
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中国植物病理学会2012年学术年会
论文部分内容阅读
Spontaneous variants of Beet black scorch virus (BBSV) and its satellite RNA were generated from cDNA clones by serial propagation in Chenopodium amaranticolor and Nicotiana benthamiana.Inoculation with recombinant RNAs synthesized in vitro revealed BBSV variants with divergent infectious phenotypes that affected either symptom expression or replication of satellite RNA variants.Sequence alignments showed a correlation between the phenotypes and distinct BBSV genomic loci in the 3 UTR or in the domain encoding the viral replicase.
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