【摘 要】
：
To establish a LC-MS method to determine [Pyr1]Apelin13 in human plasma.Plasma protein was sediment by trichloroaceticacid and analysis was performed on a UPLC-TOF-MS that was operated in the multirea
【机 构】
：
Taishan Medical College, Taian 271016 Jinin Medic
【出 处】
：
中国神经科学学会第九届全国学术会议暨第五届会员代表大会
论文部分内容阅读
To establish a LC-MS method to determine [Pyr1]Apelin13 in human plasma.Plasma protein was sediment by trichloroaceticacid and analysis was performed on a UPLC-TOF-MS that was operated in the multireaction monitoring mode using positive ion electrospray ionization (ESI) as the interface.The monitored ion was m/z 766.7.Chromatography was carried out on an WATERS ACQUITY UPLC BEH C18 (2.1 × 100 mm, 1.7 μm) column with mehanol and formate as the mobile phase at the flow rate of 0.3 ml/min, and injection volume is 30 μl, column temperature is 45 ℃.The calibration curve was linear over the range of 5-200 μg/L and the limitation of detection and quantification for Apelin was about 1 μg/L and 5 μg/L respectively; The extraction recoveries of Apelin 13 spiked at concentrations of 5, 25, 100 μg/L was 96.04%-106.3% with RSD 1.5%-2.23% (n=6).This method is simple, accurate, reliable and suitable for the determination of [Pyr1]Apelinl 3 in plasma.
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