5-腺苷磷酰硫酸激酶及R68K突变体磷酸化5-腺苷一磷酸的初步研究

来源 :第十一届全国酶学学术讨论会暨邹承鲁诞辰90周年纪念会 | 被引量 : 0次 | 上传用户:VIPT250
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  硫作为生命活动的必需元素,主要以-2和+6价发挥生物学功能.硫酸盐的活化是胞内同化代谢的关键反应之一,包括ATP硫酸化酶(ATP sulfurylase,ATPS)催化合成腺苷-5-磷酰硫酸(adenosine 5-phosphosulfate,APS)和腺苷-5-磷酰硫酸激酶(adenosine 5-phosphosulfate kinase,APSK)催化合成3-磷酸-腺苷-5-磷酰硫酸(3-phosphoadenosine 5-phosphosulfate,PAPS).APSK催化APS磷酸机理已经较为清楚,但是否可催化APS类似物AMP的磷酸化,尚无相关报道.
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