A molecular imprinted polymer (MIP) using D-tryptophan as a template and methacrylic acid (MAA) and acrylamide (AM) as a functional co-monomer was prepared.Water and methanol were used as the hydrophilic porogen with ethylene glycol dimethacrylate (EGDMA) as the crosslinker and 2,2-azobis(isobutyronitrile) (AIBN) as the initiator.Comparison of D and L form tryptophan adsorption isotherm on molecular imprinted polymer was made by the static method.The experimental parameters in the equilibrium isotherms were estimated by linear and nonlinear regression analyses.The linear equation for concentration and sorbents to adsorption concentrations was then expressed,and the adsorption equilibrium data were correlated into Langmuir,Freundlich,quadratic,Allometric,Langmuir,and Freundilich extension isotherm models.In this manner,it is possible to predict the individual band profile of separated sample components under various conditions and to optimize the separation conditions [1].The success of experiments and modeling are directly related to the accuracy of the adsorption isotherms and their parameters [2,3].The calculated data is well fitted with the experimented data.The D-tryptophan imprinted polymer showed extraordinarily higher adsorption ability for D-tryptophan than the L-tryptophan compounds that were also assessed.Furthermore,the competitive Langmuir isotherms were applied to the mixture compounds of D and L form tryptophan.