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Hyperhomocysteinemia(HHcy)is associated with liver diseases such as fatty liver and hepatic fibrosis; however,the underlying mechanism is still largely unknown.The current study aimed to explore the signaling pathway involved in HHcy-induced hepatic steatosis.C57BL/6 mice were fed a high-methionine diet(HMD)for 4 and 8 weeks to establish the HHcy mouse model.