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The successful establishment of stem cell-based therapies requires multipotent,immunocompatible stem cells, highly efficient strategies for direct differentiation, and most importantly,well-optimal culture conditions for large-scale expansion of such cell populations.Here we developed a novel chemically defined medium (CDM) combined with different group of growth factors for short-term cultivation of human menstrual blood-derived stem cells (hMBSCs).After cultivated for four to five days,MBSCs maintained their original fibroblastic morphology, and showed great proliferative potential but no considerable change in the expression of CD29, CD34, CD45 and CD166.Furthermore, in vitro migration assay using Transwell filters demonstrated that hMBSCs had great migration potential after cultivation in this CDM for four or five days.When transplanted into animal models with myocardial infarction, hMBSCs pretreated in CDM showed great migration potential into various tissues including spleen, lung and heart of peri-infarcted zones.This increase of migration potential in hMBSCs may be due to the increase of expression of MMP-2 and SDF-1 α.