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In this study,Bt-Cry1Aa gene was transformed to Populus deltoides × P.euramericana cv?Nanlin895? using Agrobacterium-mediated method.Leaves of four transgenic clones B1,B4,B17,and B21 were exposed to the larvae of Micromelalopha troglodyta to assess the insecticidal activity of Bt poplar under laboratory and field conditions.