@@Recently, multiplex immunoassay of the analytes such as biomarkers and drugs has received extensive attention because it provides the advantages in terms of higher sample throughput, shorter analysis time, less sample consumption and lower cost. So far, two dominant modes including spatial resolution and multilabel have been often adopted to perform multiplex immunoassay. However, the methods based on spatial resolution with various detection techniques are often require expensive array detectors for spatial resolution of different immunoreaction areas, while multilabel mode involves in limited labels available. So the multiple immunoassays with single-label remain a great challenge in analytical chemistry.