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目的 :研究用冻融的自体胃癌抗原冲击树突状细胞 (DC)来诱导肿瘤引流区淋巴结 (TDLN)细胞对胃癌细胞系的体外杀伤作用。方法 :采用胃癌患者外周血粘附细胞 (PBAC) ,经GM CSF、IL 4、TNF α诱导和自体肿瘤冻融抗原 (Ag)刺激诱生所获得的DC与TDLN细胞 1∶5 0比例共培养 3天 ,获得DC激活的TDLN ,即DC TDLN作效应细胞 ;分别以Ag和培液代替DC同样培养TDLN ,即Ag TDLN和TDLN作对照 ,对胃癌细胞系KATO3和黑色素瘤细胞系A375进行杀伤。结果 :DC TDLN、Ag TDLN、TDLN各组细胞对KATO3,均有显著杀伤活性 ,其中DC TDLN组的杀伤作用明显优于后两组 ,且效靶比 2 0∶1的杀伤率优于 10∶1,显示可能有量效关系 ;而TDLN各组不同效靶比对A375杀伤率则无显著差异。结论 :自PBAC获得的DC ,经自身肿瘤Ag冲击并与自身TDLN共培养 ,可使后者对胃癌细胞系细胞的杀伤作用明显增强。
OBJECTIVE: To study the in vitro killing effect of tumor-infiltrating lymph node (TDLN) cells on gastric cancer cell lines by using freeze-thawed autologous gastric cancer antigen-pulsed dendritic cells (DCs). Methods: Peripheral blood adherent cells (PBACs) from gastric cancer patients were co-cultured with GM-CSF, IL-4 and TNFα and induced with autologous tumor freeze-thaw antigen (Ag) 3 days to obtain DC-activated TDLN, DC TDLN as effector cells; respectively, Ag and culture instead of DC also cultured TDLN, Ag TDLN and TDLN as a control, the gastric cancer cell line KATO3 and melanoma cell line A375 killing. Results: The killing activity of KT03 in DC TDLN, Ag TDLN and TDLN groups was significantly higher than that in DC TDLN group, and the kill ratio of DCTLN group was better than that of 20 TDC 1, indicating that there may be a dose-effect relationship; however, no significant difference was found between the effective target ratios of the TDLN groups and the A375 killing rate. CONCLUSION: The DC obtained from PBAC can be significantly enhanced by its own tumor Ag and co-cultured with its own TDLN.