【摘 要】
：
The objective of this study was to determine the seroprevalence,risk factors and genotypes of Toxoplasma gondii in white yaks(Bos grunniens)in China.A total of 974 yak serum samples were collected fro
【机 构】
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State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of
【出 处】
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中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会
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The objective of this study was to determine the seroprevalence,risk factors and genotypes of Toxoplasma gondii in white yaks(Bos grunniens)in China.A total of 974 yak serum samples were collected from Tianzhu Tibetan Autonomous County(TTAC),Gansu province,northwest China for detecting T.gondii antibodies by the modified agglutination test(MAT),and 414 tissues belonging to 138 white yaks were collected for detecting T.gondii DNA by amplification of B1 gene with a semi-nested PCR.155 serum samples(15.91%,95%CI=1 3.62-18.21)were seropositive for T.gondii antibodies at a 1:100 cut-off,and 10 DNA samples(7.25%,95%CI=2.92-11.57)were positive for the T.gondii B1 gene,which were genetically characterized using multilocus PCR-RFLP.Only two showed complete genotyping results(ToxoDB#9).Statistically significant differences were not observed between T.gondii seroprevalence and gender,season or pregnancy in the logistic regression analysis(P>0.05).Ages of white yaks was considered as a main risk factor associated with T.gondii infection.Our results indicated a widespread exposure to T.gondii among white yaks,and revealed the genotype ToxoDB#9 of T.gondii in white yaks for the first time in China.
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