Fluorescence imaging has revolutionized biomedical research over the past three decades.However,for in vivo applications,one of its major limitations is the superficial imaging depth as random scattering in biological tissues causes exponential attenuation of the ballistic component of a light wave.Here we present two technologies to achieve deep tissue imaging.One is the focal modulation technique that takes advantage of the modulation and demodulation to enhance ballistic signal detection capability thus to obtain high resolution imaging inside deep tissues.The other is ultrasound‐pulse‐guided digital phase conjugation technique which uses scattering light to achieve fluorescence imaging beyond the ballistic regime.These technologies pave the wave for many important applications in both fundamental biology research and clinical studies.