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The activation of the NR2B and NR2A subunits of the NMDA receptor is critical for the induction of cingulate LTP, re gardless of the induction protocol.Next, in vitro whole-cell patch-clamp recordings revealed a significant enhancement in neurotransmitter release probability in anterior cingulate cortex (ACC) synapses from mice with chronic pain induced by hind paw injection of complete Freunds adjuvant (CFA).